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KMID : 0613820100200010077
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Journal of Life Science
2010 Volume.20 No. 1 p.77 ~ p.81
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Optimal Condition to Produce Protease by Strain Separated from the Intestine of Reticulitermes speratus
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Park Min-Kyung
Son Hong-Joo
Kim Yong-Gyun
Lee Sang-Mong
Kim Keun-Ki
Park Hyean-Cheal
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Abstract
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We separated the bacteria showing protease activity from Reticulitermes speratus which is known as the only termite species in Korea. Then, we collected the best activated strain and studied the optimal culture condition for producing the enzyme. According to the results of observing morphological and physiological characteristics, and the type of 16S rRNA of the strain, it was identified as Serratia marcescens and named S. marcescens strain TM-3215. This strain showed the best activity under conditions of 0.8% (w/v) starch, 0.4% (w/v) peptone, 0.08% (w/v) MgSO₄?7H₂O, 30℃ and pH 8.0. After being cultivated under optimal conditions for 9 hr, the strain produced 19.8 U/ml of enzyme, an amount 1.8 times greater than the control.
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KEYWORD
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Reticulitermes speratus, Serratia marcescens, enzyme, termite, protease
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